Contained in this excel file:   

 

The Agilent Array design: 

Probes were generated for 361 mouse ncRNAs by tiling across each transcript, including 100 nucleotides of flanking sequence extracted from genomic sequence.  Probes complementary to flanking sequences are in upper case, and annotated transcripts are in lower case. 

 

The Data: 

The Data file is the arcsinh (analogous to log, except that it allows zeros and negatives which arise from spatial detrending and cross-slide normalization) of absolute scanner intensity counts across 18 tissues (10 slides; brain and liver were duplicated in fluor reversal).  The data was spatially detrended and Huber-normalized as described in the paper.  Testis data was excluded from the published dataset as that particular microarray hyb had abnormal background levels which may have confounded the data, leaving 17 tissues/organs.

 

The miRNA data file contains only the miRNA expression data.  Several of the more recent miRNA profiles were   measured on another array and appended. The normalization was implemented to account for this (i.e. both datasets had identical   negative control intensity distributions). Non-redundant sequences with significant expression in at least one tissue. Where applicable, more recent miRNA registry annotation was implemented   (e.g. -as becomes 3p, -s becomes 5p, redundant miRNAs were eliminated etc.